Anti-DKK1 antibody ab61275 from Abcam

Product Specs
ItemAnti-DKK1 antibody
CompanyAbcam
Price Supplier Page View Supplier Product Page
Catalog Numberab61275
Antibody TypePrimary
Quantity100 UG
HostMouse, Nude mouse
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
ClonalityMonoclonal
IsotypeIgG2b
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameDKK1
ReactivityHuman
TargetDKK1

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Tail suspension delays ectopic ossification in proteoglycan‑induced ankylosing spondylitis in mice via miR‑103/DKK1Experimental and Therapeutic MedicineJuly 7, 2021Zhenzhen Zhang, Jing Zeng, Yang Li, Qing Liao, Dongdong Huang, Yucong Zou, Gang Liubuffer (Perkin-Elmer, Inc.) at 37˚C for 30 min and stained with the anti-DKK1 antibody (1:200; cat. no. ab61275, Abcam) for 1 h at room temperature. The sections were washed and incubated with HRP-coupled goat anti-mouse

(2) Downregulation of miR‑146a inhibits osteoporosis in the jaws of ovariectomized rats by regulating the Wnt/β‑catenin signaling pathwayInternational Journal of Molecular MedicineDecember 30, 2020Hua Liu, Xianhu Yue, Gang Zhangosteoprotegerin (OPG; 1:300, ab203061; Abcam), TRAP (1:10,000, ab133288; Abcam), dickkopf1 (DKK1; 1:2,000, ab61275; Abcam), Wnt2 (1:1,000, ab27794; Abcam), β-catenin (1:8,000, ab32572, Abcam) and β-actin (1:2,000, ab61275;

(3) Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathwayMolecular Medicine ReportsSeptember 25, 2020Lei Huang, Qiong Li9562; Cell Signaling Technology, Inc.), Dickkopf-related protein 1 (DKK1) (1:1,000; 29 kDa; cat no. ab61275; Abcam) and AXIN2 (1:1,000, cat no. ab109307, Abcam); GAPDH served as an internal reference. The membranes

(4) TNF‑α treatment increases DKK1 protein levels in primary osteoblasts via upregulation of DKK1 mRNA levels and downregulation of miR‑335‑5pMolecular medicine reportsAugust 1, 2020Li S, Yin Y, Yao L, Lin Z, Sun S, Zhang J, Li X.manufacturer's instructions. Mouse monoclonal anti-DKK1 antibody was purchased from Abcam (1:1,000; product code ab61275), and mouse monoclonal anti-β-actin antibody was purchased from OriGene Technologies, Inc. (1:1,000;

(5) Local Wnt3a treatment restores bone regeneration in large osseous defects after surgical debridement of osteomyelitisJournal of Molecular MedicineJune 1, 2020Johannes Maximilian Wagner et al.Danvers, USA), RANKL, Runx2, DKK1, sclerostin, sFRP1, and osteoprotegerin (cat.nr. ab45039, ab23981, ab61275, ab63097, ab4193, ab183910) (Abcam, Cambridge, UK) overnight at 4 °C, followed by washing and incubation

(6) Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathwayBMC CancerDecember 1, 2019Xiaomin Tang, Yuxuan Sun, Ganglun Wan, Jiaqiang Sun, Jingwu Sun, Chunchen PanAntibody,WB Antibody,WB Antibody,WB Antibody,WB Antibody YAP,1:400,BM4253,BOSTER,200μg/ml DDK1,1:1000,ab61275,Abcam,1 mg/ml GSK-3β,1:1000,"12,456 T",CST,239μg/ml β-catenin,1:1000,8480 T,CST,239μg/ml vimentin,1:1000,5741

(7) Stromal fibroblast activation protein alpha promotes gastric cancer progression via epithelial-mesenchymal transition through Wnt/ β-catenin pathwayBMC cancerNovember 12, 2018Liu J, Huang C, Peng C, Xu F, Li Y, Yutaka Y, Xiong B, Yang X.performed as described previously [18]. Specific primary antibodies used were the following: DKK1 (ab61275, Abcam); LEF1 (ab217378, Abcam); ZO-1 (61–7300, Thermo); Vimentin (ab92547, Abcam); N-cadherin (ab76011,

(8) MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonistsNature CommunicationsDecember 1, 2017Cong Lv et al.Cell Signaling Technology), ITGA2 (1:800, ab133557, Abcam), ITGB1 (1:800, ab52971, Abcam), Dkk1 (1:500, ab61275, Abcam). Whole mount staining Inguinal mammary glands were spread on glass slides, fixed in Carnoy’s

(9) GCN5 modulates osteogenic differentiation of periodontal ligament stem cells through DKK1 acetylation in inflammatory microenvironmentScientific reportsMay 24, 2016Li B, Sun J, Dong Z, Xue P, He X, Liao L, Yuan L, Jin Y.anti-ALP (#ab65834), anti-SP7 (#ab187158), anti-GSK3β (#ab2602), anti-β-Catenin (#ab32572), anti-DKK1 (#ab61275), anti-H3K9Ac (#ab10812), anti-H3K14Ac (#ab52946) (Abcam, Cambridge, UK), anti-GCN5 (#sc-365321, Santa Cruz Biotechnology

(10) MicroRNA-335-5p inhibits osteoblast apoptosis induced by high glucoseMolecular medicine reportsMay 1, 2016Li J, Feng Z, Chen L, Wang X, Deng H.albumin at 37˚C for 2 h, the membrane was incubated with mouse monoclonal anti‑DKK1 antibody (cat. no. ab61275; Abcam, Cambridge, UK; 1:200 dilution), rabbit polyclonal anti‑caspase‑3 antibody (cat. no. 9662; Cell Signaling Technology

(11) Dickkopf-1 promotes hyperglycemia-induced accumulation of mesangial matrix and renal dysfunctionJournal of the American Society of Nephrology : JASNJanuary 1, 2010Chun-Liang Lin, Jeng-Yi Wang, Jih-Yang Ko, Yu-Ting Huang, Yu-Hsia Kuo, Feng-Sheng Wang

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Anti-DKK1 antibody from Abcam

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Anti-DKK1 antibody from Abcam

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